ABSTRACT
Background: the role of Matrix Metalloproteinase 9 [MMP9] in tumor invasion and progression is prominent. A single nucleotide polymorphism [SNP] in the promoter region of MMP9 [-1562 C/T] increases the transcription and expression of this gene. On the other hand, MHC class I chain-related protein A and B [MICA/B] in soluble forms may impair tumor immunogenicity by reducing Natural Killer Group 2D [NKG2D] densities on NK cells and MMP9 enzyme activity has a prominent role in shedding of MICA/B
Objectives: to investigate the association between MMP9 [-1562 C/T] polymorphism and serum MICA/B level in breast cancer patients
Methods: in this case-control study, 105 patients with breast cancer and 100 healthy age-matched women were selected from Yazd hospitals, Iran. The polymorphism of MMP9 [-1562 C/T] was determined by PCR-RFLP. Concentration of MICB and MICA in the sera of breast cancer patients and healthy women were measured using ELISA method
Results: the frequency of CC, CT and TT genotypes and T allele of the MMP9 [-1562 C/T] did not show significant differences between breast cancer patients and healthy donors [p>0.05]. On the other hand, the mean serum levels of MICB and MICA were significantly elevated in patients compared with healthy individuals [p<0.05]. In patients with MMP9CC genotype, the mean serum MICB concentration was significantly higher than those patients with CT polymorphism [p<0.05]. Although the mean of blood MICA concentration in patients with the CT genotype was higher than those patients with CC genotype, the difference was not statistically significant
Conclusion: the T allele of the MMP9 [-1562 C/T] does not show a correlation with serum levels of MICA and MICB in breast cancer patients
ABSTRACT
Mycobacterium tuberculosis lipid antigens take part in pathogenicity of the bacterium but the response of monocytes/macrophages to these antigens in tuberculosis is not well known. The aim of current investigation was to study the M. tuberculosis lipid antigens in tuberculosis pathogenesis. In the present study M. tuberculosis lipid antigens were extracted. Monocytes and macrophages from multidrug-resistant tuberculosis [MDR-TB], TB patients, asymptomatic healthy individuals with positive tuberculin skin test positive and healthy individuals with negative tuberculin skin test were collected using magnetic cell sorting. The cells were stimulated by M. tuberculosis total lipid antigens and IL-12 and IL-10 in their supernatants were measured by enzyme-linked immunosorbent assay. The IL-12 production by monocytes in response to M. tuberculosis total sonicate antigens in the MDR-TB patients did not show a considerable difference with the PPD positive healthy subjects, whereas in the active TB patients, IL-12 levels significantly decreased [p<0.05]. IL-10 production by monocytes in TB patients in response to total lipid antigens showed a significant increase in comparison to MDR-TB patients and healthy individuals. In the MDR-TB patients, IL-10 and IL-12 production by monocytes in response to M. tuberculosis lipid antigens are similar to the healthy subjects
Subject(s)
Humans , Male , Female , Mycobacterium tuberculosis/immunology , Antigens, Bacterial , Interleukin-10 , Interleukin-12 , Lipids/immunology , Macrophages , MonocytesABSTRACT
CD4 [+]T-cell have a central role in protective immune responses to Mycobacterium tuberculosis [M. tuberculosis] protein antigens, but function of these cells in response to M. tuberculosis total lipid antigens has remained unclear. The present study was undertaken to determine role of CD4[+] T cells in the MDR-TB patients against M. tuberculosis total lipid antigens. CD4[+]T- cells were isolated from MDR-TB patients and stimulated with M. tuberculosis total lipid antigens. Proliferative responses and cytokine secretion were assessed by MTT and ELISA, respectively. Our study results showed that proliferative responses of stimulated CD4[+]T- cells to M. tuberculosis total lipid antigens and IFN-gamma production in MDR-TB patients were significantly lower than those of the PPD-positive subjects [P < 0.05] whereas, IL-4 production in the MDR-TB patients was elevated[P < 0.05]. Responses of CD4[+]T -cells of MDR-TB patients to total lipid antigens was significantly lower than that of PPDpositive healthy subjects. Therefore, it seems that M. tuberculosis lipid antigens, as protein antigens, have an important role in specific immune response